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众所周知,很多妊娠病例羊水和母体血清中胎甲球(AFP)升高伴有神经管缺陷(NTD)及某些其他胎儿畸形。进一步证明几乎所有的神经管缺陷的妊娠羊水中,乙酰胆碱脂酶(AChE)的浓度升高,但母体血清AChE是否象AFP相应增加尚待研究。过去由于既无痕量对AChE绝对特异的酶的底物、也无抑制物、又无丰富的酪基胆碱脂酶(BChE)存在,所以不可能可靠的测定血清中的AChE。而现在已有可行血清中AChE的定量分析的免疫化学方法。本工作目的是测定胎儿血清、羊水和母体血清中AChE的发生以及研究是否母体血清中AChE的测定可用于NTD的产前诊断。对于胎儿畸形通过AFP筛选程序,从妊娠妇
It is well known that many pregnancies, including amniotic fluid and maternal serum fetal nuclei (AFP), are elevated with neural tube defects (NTDs) and some other fetal abnormalities. Further evidence that almost all neural tube defects in amniotic fluid of pregnant women with elevated levels of acetylcholinesterase (AChE), but whether maternal serum AChE increases correspondingly to AFP is yet to be studied. In the past, it was impossible to reliably measure AChE in serum because there was not a trace amount of substrate for enzyme absolutely specific to AChE, neither inhibitor nor abundant cholestyroperase (BChE). However, immunochemical methods for the quantitative analysis of AChE in serum are now available. The aim of this work was to determine the occurrence of AChE in fetal serum, amniotic fluid and maternal serum and to investigate whether the determination of AChE in maternal serum can be used for prenatal diagnosis of NTD. AFP screening program for fetal malformations from pregnant women