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目的:探讨尼美舒利对人γδT细胞功能影响。方法:按常规方法培养γδT细胞,收集培养第9天的γδT细胞用不同浓度的尼美舒利(分别为0.25、0.5、1、2、4μmol/L)诱导24 h后收集培养上清液检测细胞因子IFN-γ、TNF-α、IL-12,沉淀细胞流式细胞测定穿孔素、粒酶B和NKG2D,LDH法检测γδT细胞杀伤胃癌细胞活性。结果:经尼美舒利诱导后γδT细胞穿孔素、粒酶B表达量(分别为62.8%和72.7%)明显高于对照组(分别为51.4%和60.9%)P<0.05,尤其尼美舒利浓度在1μmol/L时最显著;经尼美舒利诱导后γδT细胞分泌IFN-γ和TNF-α浓度(分别为262.3 ng/L和177.5 ng/L)明显高于对照组(分别为196.1 ng/L和158.5 ng/L)P<0.05;分泌的IL-12浓度与对照组比较无明显差异(P>0.05)。γδT细胞杀伤胃癌细胞SGC-7901和BCG-823的活性在1μmol/L时最高(分别为73%和70%),明显高于对照组(分别为54%和53%)P<0.05。结论:经尼美舒利诱导后γδT细胞的穿孔素、粒酶B含量和γδT细胞杀伤胃癌细胞SGC-7901、BCG-823活性明显高于对照组。其培养上清液中IFN-γ和TNF-α浓度也明显高于对照组。这一结果为临床尼美舒利预防和治疗消化道肿瘤提供了实验依据。
Objective: To investigate the effect of nimesulide on the function of human γδT cells. Methods: γδT cells were cultured by conventional methods. The γδT cells collected on the 9th day were collected and cultured for 24 hours with different concentrations of nimesulide (0.25, 0.5, 1, 2, 4μmol / L) Perforin, granzyme B and NKG2D were determined by flow cytometry of cytokines IFN-γ, TNF-α, IL-12 and precipitated cells, and the activity of γδT cells was detected by LDH assay. Results: The expression of perforin and granzyme B in γδT cells induced by nimesulide (62.8% and 72.7%, respectively) was significantly higher than that in the control group (51.4% and 60.9%, P <0.05), especially nimesulide The concentration of IFN-γ and TNF-α in γδT cells induced by nimesulide (262.3 ng / L and 177.5 ng / L, respectively) was significantly higher than that of the control group (196.1 ng / L and 158.5 ng / L), P <0.05. The secreted IL-12 concentration had no significant difference compared with the control group (P> 0.05). The activity of γδT cells killing SGC-7901 and BCG-823 cells was the highest at 1μmol / L (73% and 70%, respectively), which was significantly higher than that of the control group (54% and 53%, P <0.05). CONCLUSION: The results showed that the activity of SGC-7901 and BCG-823 was significantly higher in γδT cells induced by nimesulide than in control group. The concentration of IFN-γ and TNF-α in the culture supernatant was also significantly higher than that of the control group. This result provides an experimental basis for clinical nimesulide prevention and treatment of gastrointestinal tumors.