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已研究出—种具有高特异活性的制备鲑降钙素(Salmon calcitonin)长效处方的新方法。该方法包括两个过程:1)鲑降钙素高分子量衍生物的制备;b)含锌衍生物混悬剂的制备。衍生物制备是在0.1M 磷酸盐缓冲液中(pH 8.2),用二硫苏糖醇还原鲑降钙素,并用氯胺T 方法进行碘标记。已还原的鲑降钙素经葡聚糖凝胶层析柱分离后冻干。用0.1M 醋酸洗脱。然后,将已还原的鲑降钙素用含1%牛血清白蛋白的0.1M 磷酸盐缓冲液(pH8.2)溶解,置60C 保温10min
A new method for the preparation of long-acting formulations of salmon calcitonin with high specific activity has been developed. The method comprises two processes: 1) preparation of salmon calcitonin high molecular weight derivatives; b) preparation of zinc-containing derivative suspensions. Derivatives were prepared by reducing salmon calcitonin with dithiothreitol in 0.1 M phosphate buffer (pH 8.2) and iodine labeling with the chloramine T method. The reduced salmon calcitonin is separated on a Sephadex column and lyophilized. Elute with 0.1 M acetic acid. Then, the reduced salmon calcitonin was dissolved in 0.1 M phosphate buffer (pH 8.2) containing 1% bovine serum albumin and incubated at 60 C for 10 min