建立了用离子交换层析法测定人及动物尿中三甲基硒离子(TM-Se~+)的分析方法,方法的精密度及准确度良好,可以用于不同硒状态尿中三甲基硒离子的测定。给大鼠注射大剂量亚硒酸钠后,在一定剂量下,TMSe~+的排出率随剂量的增加而上升,超过剂量后则变化不大。测定高硒地区居民尿中TMSe~+后,发现尿中TMSe~+-Se的浓度和排出量均相应的随尿硒(T-Se)浓度及总排出量的增加而增加,但TMSe~+-Se/T-Se比率的增加只限于尿中T-Se排出量在80μg以下的12小时夜尿样品。 The analytical method for the determination of TM-Se ~ + in urine of human and animal urine by ion exchange chromatography was established. The method has good precision and accuracy and can be used in the determination of trimethyl Determination of selenium ions. After the rats were injected with large doses of sodium selenite, the discharge rate of TMSe ~ + increased with the increase of dose at a certain dose, but little changed after the dose was exceeded. The concentration of TMSe ~ + -Se in urine and the discharge of urinary TMSe ~ + in the urine of high-selenium area residents were increased correspondingly with the increase of urinary selenium (T-Se) concentration and total discharge, but TMSe ~ + The increase in the -Se / T-Se ratio was limited to 12-hour nocturia samples with urinary T-Se excretion less than 80 μg.