Mucoepidermoid carcinoma undergoes uniquely vigorous angiogenic and neovascularization processes,possibly due to proliferation of vascular endothelial cells(ECs) induced by mucoepidermoid carcinoma cells(MCCs) in their three-dimensional(3D) microenvironment.To date,no studies have dealt with tumor cells and vascular ECs from the same origin of mucoepidermoid carcinoma using the in vitro 3D microenvironment model.In this context,the current research aims to observe neovascularization with mucoepidermoid carcinoma microvascular ECs(MCMECs) conditioned by the microenvironment in the 3D collagen matrix model.We observed the growth of MCMECs purified by immunomagnetic beads and induced by MCCs,and characteristics of tubule-like structures(TLSs) formed by induced MCMECs or non-induced MCMECs.The assessment parameters involved the growth curve,the length,the outer and inner diameters,and the wall thickness of the TLSs,and the cell cycle.Results showed that MCCs induced formation of the TLSs in the 3D collagen matrix model.A statistically significant difference was noted regarding the count of TLSs between the control group and the induction group on the 4th day of culture(t=5.00,P=0.001).The outer and inner diameters(t1=5.549,P1=0.000;t2=10.663,P2=0.000) and lengths(t=18.035,P=0.000) of the TLSs in the induction group were statistically significant larger than those in the control group.The TLSs were formed at the earlier time in the induction group compared with the control group.It is concluded that MCCs promote growth and migration of MCMECs,and formation of the TLSs.The 3D collagen matrix model with MCMECs induced by MCCs in the current research may be a favorable choice for research on pro-angiogenic factors in progression of mucoepidermoid carcinoma. Mucoepidermoid carcinoma undergoes uniquely vigorous angiogenic and neovascularization processes, possibly due to proliferation of vascular endothelial cells (ECs) induced by mucoepidermoid carcinoma cells (MCCs) in their three-dimensional (3D) microenvironment. To date, no studies have dealt with tumor cells and vascular ECs from the same origin of mucoepidermoid carcinoma using the in vitro 3D microenvironment model. in this context, the current research aims to observe neovascularization with mucoepidermoid carcinoma microvascular ECs (MCMECs) conditioned by the microenvironment in the 3D collagen matrix model. growth of MCMECs purified by immunomagnetic beads and induced by MCCs, and characteristics of tubule-like structures (TLSs) formed by induced MCMECs or non-induced MCMECs.The assessment parameters involved the growth curve, the length, the outer and inner diameters, and the wall thickness of the TLSs, and the cell cycle. Results showed that MCCs induced formation of the TLSs i The 3D collagen matrix model. A significant significant difference was noted regarding the count of TLSs between the control group and the induction group on the 4th day of culture (t = 5.00, P = 0.001). The outer and inner diameters (t1 = T = 18.035, P = 0.000) of the TLSs in the induction group were significantly significant than those in the control group. TLSs were formed at the earlier time in the induction group compared with the control group. It is said that MCCs promote growth and migration of MCMECs, and formation of the TLSs.The 3D collagen matrix model with MCMECs induced by MCCs in the current research may be a favorable choice for research on pro-angiogenic factors in progression of mucoepidermoid carcinoma.