论文部分内容阅读
目的通过对海产品副溶血性弧菌(vibrio parahaemolyticus,VP)血清群、毒力基因、耐药性检测,了解三亚市海产品VP污染程度及血清群分布、毒力基因和耐药状况。方法参照GB/T4789.7-2008规范检验,采用实时荧光定量PCR方法检测毒力基因,K-B琼脂纸片扩散法做药敏试验。结果 208份海产品检出60份,分属9个血清群,所有VP均未携带tdh、trh,全携带tlh。38份患者分离株分属4个血清群,全携带tlh,未携带trh,36株携带tdh,占94.74%。76株药敏结果显示,对氨基糖苷类和喹诺酮类抗生素高度敏感。结论三亚市海产品VP血清群分布多样性,贝壳类受VP污染较严重,对氨基糖苷类和喹诺酮类抗生素高度敏感;海产品VP均未携带tdh和trh,患者分离株以O4、O3血清群为主,患者与海产品分离株关联性不大,需继续加强对VP监测及耐药性研究。
Objective To understand the VP pollution level, serogroup distribution, virulence genes and drug resistance in seafood of Vibrio parahaemolyticus (VP) in China by detecting the serogroups, virulence genes and drug resistance of marine products. Methods Reference GB / T4789.7-2008 standard test, using real-time fluorescent quantitative PCR method to detect virulence genes, K-B agar disk diffusion method for drug susceptibility testing. Results 208 seafood samples were detected in 60, belonging to 9 serogroups, all VP did not carry tdh, trh, all carrying tlh. 38 isolates belonged to 4 serogroups, all carrying tlh, not carrying trh, 36 carrying tdh, accounting for 94.74%. 76 strains of susceptibility results showed that aminoglycosides and quinolone antibiotics are highly sensitive. Conclusion The distribution of VP serogroups in marine products in Sanya City is quite diverse. Shellfish are more polluted by VP and highly sensitive to aminoglycosides and quinolone antibiotics. Tdh and trh are not carried in seafood VPs. O4 and O3 serogroups Mainly associated with patients with seafood isolates, the need to continue to strengthen the VP monitoring and drug resistance.