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目的:探讨硒对氟致小鼠成釉细胞DNA损伤的拮抗作用。方法:小鼠成釉细胞未经氟化钠处理为对照组;以氟化钠浓度分别为0.25,0.50,1.00,2.00,4.00mmol/L处理作为单独染氟组;以2.50,5.00,10.00μmol/L Na2SeO3为单独染硒组;以2.50,5.00,10.00μmol/L Na2SeO3+0.25,0.50,1.00,2.00,4.00mmol/L氟化钠为联合作用组。细胞作用24h后,采用单细胞凝胶电泳技术(SCGE)检测DNA单链断裂情况。结果:与对照组相比,单独染硒组小鼠成釉细胞的尾长、Olive尾矩、尾DNA%、尾长/头长值显著性升高(P<0.05),而单独染硒组以上指标均明显下降(P<0.05)。2.50,5.00,10.00μmol/L Na2SeO3+氟化钠联合作用小鼠成釉细胞的尾长、Olive尾矩、尾DNA%、尾长/头长值明显低于对照组和相同剂量单独染氟组(P<0.05),且高于相应剂量的单独染硒组(P<0.05)。与2.50μmol/L Na2SeO3+氟化钠联合染毒组比较,5.00,10.00μmol/L Na2SeO3+各剂量氟化钠联合作用组Olive尾矩均升高(P<0.05)。结论:过量摄入氟化物可导致小鼠成釉细胞DNA损伤,而适量的硒对氟致DNA损伤有一定的拮抗作用,且实验浓度为2.50μmol/L时其拮抗效果较好。
Objective: To explore the antagonistic effect of selenium on DNA damage induced by fluoride in ameloblasts of mice. Methods: Mouse ameloblasts without sodium fluoride as control group; sodium fluoride concentrations were 0.25,0.50,1.00,2.00,4.00 mmol / L treatment as a separate group with fluorine; with 2.50,5.00,10.00μmol / L Na2SeO3 selenium group alone; 2.50,5.00,10.00μmol / L Na2SeO3 +0.25,0.50,1.00,2.00,4.00mmol / L sodium fluoride as a combined effect group. After 24 hours, single-cell gel electrophoresis (SCGE) was used to detect DNA single-strand breaks. Results: Compared with the control group, tail length, Olive tail moment, tail DNA%, tail length / head length of ameloblasts in selenium alone group were significantly increased (P <0.05) The above indicators were significantly decreased (P <0.05). The tail length, Olive tail moment, tail DNA%, tail length / head length of ameloblasts in mice treated with 2.50, 5.00, 10.00μmol / L Na2SeO3 + sodium fluoride were significantly lower than those in the control group and the same dose alone P <0.05), and higher than the corresponding dose of selenium alone group (P <0.05). Compared with the combination of 2.50μmol / L Na2SeO3 + sodium fluoride, Olive tail moment of 5.00,10.00μmol / L Na2SeO3 + combined with sodium fluoride increased (P <0.05). CONCLUSION: Excessive fluoride ingestion can cause DNA damage in ameloblasts of mice. However, moderate amounts of selenium can antagonize DNA damage induced by fluoride, and antagonistic effect is better when the concentration is 2.50μmol / L.