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目的 探讨于缺血前全身应用N 乙酰半胱氨酸 (NAC)对大鼠离体缺血 再灌注心脏的保护作用及其机制。方法 应用Langendorff离体心脏灌注模型和心功能测定程序记录左心室压力波形并作各项参数分析 ,同时检测不同时刻冠状静脉流出液中的丙二醛 (MDA)含量和超氧化物歧化酶 (SOD)活性 ,并作冠脉流量和心肌含水百分比测定。结果 研究发现NAC能够显著改善左心室血流动力学指标 ,增强心肌收缩力和舒张性 ,减轻生物膜脂质过氧化程度 (MDA含量较对照组显著减低 ) ,但心肌含水量、冠状动脉流量、冠脉流出液中SOD活性较对照组无明显改善。结论 提示NAC的心肌保护作用与加强GSH的抗氧化能力和自身的抗氧自由基作用有关 ,NAC能够对缺血 再灌注心肌起到明显的保护作用。
Objective To investigate the protective effect of NAC on systemic ischemia / reperfusion (I / R) injury in rats and its mechanism. Methods The Langendorff perfusion model and cardiac function measurement program were used to record the left ventricular pressure waveform and analyze the parameters. Meanwhile, the content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) in the coronary venous effusion ) Activity, and coronary flow and myocardial water percentage determination. Results The study found that NAC can significantly improve left ventricular hemodynamic parameters, increase myocardial contractility and relaxation, reduce the degree of lipid peroxidation (MDA content significantly lower than the control group), but the myocardial water content, coronary flow, Coronary effluent SOD activity than the control group no significant improvement. Conclusion NAC myocardial protection and enhanced GSH antioxidant capacity and its role in antioxidant free radicals, NAC can play a significant protective effect of myocardial ischemia-reperfusion.