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应用 Southern印迹杂交技术和 PCR技术,进行人 FⅧ基因内含子18/BclI、内含子22/Xbal和 Stl4/TaqI RFLPs连锁分析,FⅧ基因内含子 13(CA)。多态分析Stl4(DXS52)VNTR多态连锁分析,选择最佳联合提出甲型血友病快速基因诊断的较佳方案。
Southern blot hybridization and PCR were used to analyze the association of human FⅧ gene intron 18 / BclI, intron 22 / Xbal and Stl4 / TaqI RFLPs with FⅧ gene intron 13 (CA). Polymorphism Analysis Stl4 (DXS52) VNTR polymorphic linkage analysis, select the best combination of the proposed rapid detection of hemophilia hemoglobin gene better program.