作者介绍制备一种完整核心脂多糖(LPS)脂质体疫苗的方法,并对其进行临床前评价。作者使用了4种菌株,即大肠埃希菌(大肠杆菌)、克雷伯肺炎杆菌、铜绿假单胞菌(绿脓杆菌)、脆弱类杆菌。制备的方法是先将上述细菌经肉汤培养后收集菌体,制成冻干细胞,然后用酚法或PCP法或Triton法提取并纯化LPS。由二十四烷酸磷酸胆碱 The authors describe the preparation of a complete core lipopolysaccharide (LPS) liposomal vaccine approach, and its pre-clinical evaluation. The authors used four strains, Escherichia coli (E. coli), Klebsiella pneumoniae, Pseudomonas aeruginosa (Pseudomonas aeruginosa), Bacteroides fragilis. The preparation method comprises the following steps of: firstly, culturing the above-mentioned bacteria in the broth, collecting the cells, making lyophilized cells, and then extracting and purifying the LPS by a phenol method or a PCP method or a Triton method. From tetracosanoyl phosphorylcholine