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目的了解江西省2009年-2014年AFP病例中脊髓灰质炎病毒的分离情况,并分析Ⅰ型脊髓灰质炎病毒疫苗相关株的基因特点。方法利用分子生物学技术,通过real-time PCR对病毒分离株进行型内鉴定,并且对毒株进行VP1编码区全基因的序列测定和分析。结果 2009年-2014年,从江西省各地送检的粪便标本中,共检出9例Ⅰ型脊髓灰质炎病毒。其有相似的核苷酸序列,其核苷酸序列长度均为906 bp,编码302个氨基酸,同源性为98.6%~99.9%,与SabinⅠ序列相比,共有15个核苷酸突变位点,每株毒株的突变率为0.11%~0.99%。结论 9株均为疫苗相关株,VP1全基因编码区发现有1株Ⅰ型毒株在突变率最高的位点2 795位发生突变,可能引起其毒力回复,应加强对其进一步监测。
Objective To understand the isolation of poliovirus in AFP cases from 2009 to 2014 in Jiangxi Province and to analyze the genetic characteristics of poliovirus type Ⅰ vaccine-related strains. Methods Molecular biology techniques were used to identify the virus isolates by real-time PCR. The whole genome of VP1 was sequenced and analyzed. Results From 2009 to 2014, 9 cases of poliovirus type Ⅰ were detected in the stool samples sent from all over Jiangxi Province. It has a similar nucleotide sequence with a nucleotide sequence of 906 bp, encoding 302 amino acids and a homology of 98.6% -99.9%. Compared with the Sabin I sequence, there are 15 nucleotide mutation sites The mutation rate of each strain was 0.11% ~ 0.99%. Conclusion All of the 9 strains were vaccine-related strains. One strain of type Ⅰ was found in the coding region of VP1 gene at position 2 795, which had the highest mutation rate, which may lead to its virulence recovery. Therefore, its further monitoring should be strengthened.