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目的:探讨si RNA抑制受体相互作用蛋白激酶4(receptor-interacting protein kinase 4,RIPK 4)基因表达对人成骨肉瘤MG-63细胞增殖及细胞内Wnt/β-catenin信号通路相关基因表达的影响。方法 :将靶向RIPK4基因的si RNA(RIPK4 si RNA)转染入人成骨肉瘤MG-63细胞后,应用CCK-8法检测MG-63细胞的增殖能力,实时荧光定量PCR法和蛋白质印迹法检测细胞内RIPK4、β-catenin和cyclin D1m RNA及蛋白的表达水平。结果 :RIPK4 si RNA转染后,MG-63细胞的增殖能力显著低于阴性对照组(MG-63细胞转染control si RNA)和空白对照组(未转染任何si RNA的MG-63细胞)(P值均<0.05);RIPK4 si RNA转染组细胞中RIPK4、β-catenin和cyclin D1 m RNA及蛋白的表达水平显著低于阴性对照组和空白对照组(P值均<0.05)。结论 :沉默RIPK4基因的表达可以抑制人成骨肉瘤MG-63细胞的增殖,这一作用可能与抑制Wnt/β-catenin信号通路有关。
AIM: To investigate the effect of si RNA on receptor-interacting protein kinase 4 (RIPK 4) gene expression on the proliferation of human osteosarcoma MG-63 cells and the expression of intracellular Wnt / β-catenin signaling pathway influences. Methods: RIPK4 siRNA targeting RIPK4 gene was transfected into human osteosarcoma MG-63 cells. The proliferation of MG-63 cells was detected by CCK-8. Real-time PCR and Western blot The levels of RIPK4, β-catenin and cyclin D1m RNA and protein in cells were detected by immunohistochemistry. RESULTS: After transfected with RIPK4 si RNA, the proliferation ability of MG-63 cells was significantly lower than that of negative control group (MG-63 cells transfected with control si RNA) and blank control group (MG-63 cells without transfected with any si RNA) (P <0.05). The expression of RIPK4, β-catenin and cyclin D1 mRNA and protein in RIPK4 si RNA transfected group were significantly lower than those in negative control group and blank control group (all P <0.05). Conclusion: The silencing of RIPK4 gene can inhibit the proliferation of human osteosarcoma MG-63 cells, which may be related to the inhibition of Wnt / β-catenin signaling pathway.