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目的探讨5岁以下喘息患儿Clara细胞分泌蛋白10(CC10)基因G38A位点多态性与喘息发病机制的关系。方法随机选取于本院就诊的5岁以下反复喘息患儿120例,分为有特应质高危因素的喘息Ⅰ组(n=67)(湿疹45例,父母或父母一方有哮喘病史13例,变应性鼻炎5例,变应性皮炎4例)和无特应质高危因素的喘息Ⅱ组(n=53);对照组为本院外科近期无感染疾病史、择期进行手术的术前患儿(n=55)。采用PCR-限制性片段长度多态性分析对喘息组和对照组患儿CC10 G38A位点基因型频率和等位基因频率进行检测,比较3组间CC10 G38A位点基因型频率和等位基因频率。结果喘息Ⅰ组、喘息Ⅱ组和对照组3种基因型AA、GA、GG分布频率分别为20.9%、44.8%、34.3%,9.4%、32.1%、58.5%、9.1%、31.0%、60.0%;喘息Ⅰ组和喘息Ⅱ组CC10基因G38A位点基因型频率比较差异有统计学意义(P<0.05);喘息Ⅰ组和对照组CC10基因G38A位点基因型频率比较差异亦具有统计学意义(P<0.05)。喘息Ⅰ组、喘息Ⅱ组和对照组38A和38G等位基因频率分别为43.3%、56.7%,25.5%、74.5%,24.5%、75.5%;喘息Ⅰ组和喘息Ⅱ组、喘息Ⅰ组和对照组比较差异均有统计学意义(Pa<0.05)。结论喘息患儿与哮喘存在相同的基因分布频率,发生哮喘的危险性高;对于CC10基因具有A等位基因的喘息患儿应密切关注。
Objective To investigate the relationship between G38A polymorphism of clara cell secretory protein 10 (CC10) gene and the pathogenesis of asthma in infants with wheeze under 5 years old. Methods A total of 120 children with recurrent wheeze below 5 years of age who were admitted to our hospital were randomly divided into wheezing group Ⅰ (n = 67) (45 cases with eczema, 13 cases with asthma history from parents or parents, 5 cases of allergic rhinitis, 4 cases of allergic dermatitis) and asthmatic group Ⅱ (n = 53) without risk factors of atherosclerosis. The control group was hospital-based surgical patients with no history of infection and had preoperative elective surgery Children (n = 55). PCR-restriction fragment length polymorphism analysis of wheezy group and control group CC10 G38A locus genotype frequencies and allele frequencies were detected between the three groups CC10 G38A locus genotype frequency and allele frequency . Results The frequency of AA, GA and GG genotypes in asthmatic group Ⅰ, asthmatic group Ⅱ and control group were 20.9%, 44.8%, 34.3%, 9.4%, 32.1%, 58.5%, 9.1%, 31.0% and 60.0% (P <0.05). There was also significant difference in genotype frequencies of CC10 G38A locus between wheezing group Ⅰ and control group (P <0.05) P <0.05). The allele frequencies of 38A and 38G in asthmatic group Ⅰ, asthmatic group Ⅱ and control group were 43.3%, 56.7%, 25.5%, 74.5%, 24.5% and 75.5%, respectively; wheezing group Ⅰ and asthmatic group Ⅱ, respite group Ⅰ and control group The difference was statistically significant (Pa <0.05). Conclusion The prevalence of asthma in asthmatic children with wheezing and asthma is the same, and the risk of asthma is high. Children with asthma who have allele A of CC10 should pay close attention to them.