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目的:探讨通过流式细胞术进行食管脱落细胞DNA分析,在食管癌诊断中的价值。方法:用食管拉网法获得61例食管癌和31例非食管癌患者食管脱落细胞,记录和计算DNA指数(DI)、G2/G1、S期比率(SPF)和细胞增殖活性指数(PI)等,同时结合肿瘤的临床病理学特征,并与脱落细胞常规细胞学检查进行对比。结果:食管癌组DI为1.31±0.23,对照组为1.00±0.03;异倍体(An)出现率为70.7%,高于对照组4.8%;食管癌组G2/G1为2.071±0.153,对照组为1.996±0.099;并且SPF和PI两组指标的差异均有统计学意义,P<0.05。结论:食管脱落细胞DNA增殖活性指标对食管癌普查及提高早期诊断率具有一定的应用价值。
Objective: To investigate the value of esophageal exfoliated cell DNA analysis by flow cytometry in the diagnosis of esophageal cancer. Methods: The esophageal and esophageal cancer specimens of 61 patients with esophageal cancer and 31 patients with esophageal cancer were collected. The DNA index (DI), G2 / G1, S phase ratio (SPF) and cell proliferative activity index (PI) Etc., combined with the clinical and pathological features of the tumor, and exfoliated cells compared to conventional cytology. Results: The DI was 1.31 ± 0.23 in esophageal cancer group and 1.00 ± 0.03 in control group, and the incidence of An was 70.7% in esophageal cancer group and 4.8% in control group. The G2 / G1 in esophageal cancer group was 2.071 ± 0.153, 1.996 ± 0.099, and the differences of SPF and PI between the two groups were statistically significant (P <0.05). Conclusion: The indexes of DNA proliferation in esophageal exfoliated cells have certain value in the screening of esophageal cancer and improving the early diagnosis rate.