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目的分析野生小鼠来源1号染色体替换系小鼠群体的生长表型与血液生化指标,探讨替换系群体的QTL定位潜力。方法以染色体替换系近交后代小鼠为研究对象,测定其体重、体长、尾长、血液生化、内脏器官重量等表型数据,统计其与C57BL/6差异显著性。结果发现替换系小鼠在多个指标上与C57BL/6具差异有显著性。不同发育时期的外部表型上与C57BL/6相比,体重具显著差异的是B6-Chr1KM等6个品系,体长具显著差异的是B6-Chr1K M等5个品系,尾长具显著差异的是B6-Chr1K M等9个品系;部分品系在肝、脾、肾脏和脑部重量上存在与C57BL/6的显著差异;生化指标上,丙氨酸氨基转移酶B6-Chr1CM雌鼠显著偏高,碱性磷酸酶B6-Chr1HZ雌鼠显著偏高、B6-Chr1KM雄鼠显著偏低,总胆红素B6-Chr1CM雄鼠、B6-Chr1SMX雄鼠和B6-Chr1HZ雄鼠显著偏高,甘油三酯B6-Chr1SMX雄鼠显著偏高,总胆固醇B6-Chr1TW雄鼠显著偏高。结论培育的野生小鼠来源的1号染色体替换系群体在部分表型上与C57BL/6差异有显著性,具备作为QTL定位的遗传资源潜力。
OBJECTIVE: To analyze the growth phenotypes and blood biochemical indices of the mouse population derived from chromosome 1 replacement in wild-type mice and to explore the potential of QTL mapping for the population of replacement lines. Methods Chromosomal replacement of inbred offspring mice was used as the research object to measure the body weight, body length, tail length, blood biochemistry, visceral organ weight and other phenotypic data, statistics and C57BL / 6 significant difference. As a result, it was found that the substitution line mice showed significant differences from C57BL / 6 on multiple indicators. There were 6 strains of B6-Chr1KM with significant difference in body weight between C57BL / 6 and different phenotypes at different developmental stages. Five strains of B6-Chr1K M were significantly different in body length, with significant differences in tail length B6-Chr1K M and other nine strains; some strains in the liver, spleen, kidney and brain weight and C57BL / 6 significant differences; biochemical indicators, alanine aminotransferase B6-Chr1CM female mice significantly High, alkaline phosphatase B6-Chr1HZ female rats were significantly higher, B6-Chr1KM male mice were significantly lower, total bilirubin B6-Chr1CM male, B6-Chr1SMX male and B6-Chr1HZ male rats were significantly higher glycerol Triester B6-Chr1SMX male mice were significantly higher, total cholesterol B6-Chr1TW male mice were significantly higher. Conclusion The population of chromosome 1 replacement of cultivated wild mice was significantly different from that of C57BL / 6 in partial phenotype, which possessed the potential of genetic resources for QTL mapping.