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目的探讨模拟失重对人胃癌SGC-7901细胞形态、增殖、周期和凋亡的影响。方法采用回转器模拟失重,用图像分析系统软件分析HE染色后SGC-7901细胞形态的改变,用免疫组化法观察细胞增殖细胞核抗原(PCNA)表达的变化,用流式细胞仪测定细胞周期,并用TIJNEL法检测细胞凋亡。结果回转器模拟失重后,回转组SGC-7901细胞12 h、24 h、36 h、48 h及72 h各时间段的细胞面积均较对照组缩小(P<0．01);在36 h时,其长短径之比较对照组增大(P<0．01),其余时间段无明显差异。12 h、24 h和36 h回转组SGC-7901细胞PCNA抗体阳性细胞比率与对照组相比无明显差异,48 h、72 h回转组的PCNA抗体阳性细胞明显减少(P<0．05或P<0．01)。12 h、 24 h和36 h回转组SGC-7901细胞的细胞周期与对照组相比无明显差异,48 h、72 h回转后G0+G1 期细胞显著增多(P<0．01或P<0．05),S+G2+M期细胞则显著减少(P<0．01或P<0．05)。 12 h、24 h、36 h和48 h回转组SGC-7901细胞凋亡比例与对照组相比无明显差异,72 h回转组细胞凋亡比例较对照组明显增多(P<0．01)。结论回转器模拟失重使胃癌SGC-7901细胞的形态发生了变化,48 h、72 h时出现细胞周期阻滞、增殖抑制,72 h时凋亡增加。
Objective To investigate the effect of simulated weightlessness on the morphology, proliferation, cycle and apoptosis of human gastric cancer cell line SGC-7901. Methods The rotator was used to simulate weight loss. The morphological changes of SGC-7901 cells were analyzed by image analysis software. The expression of PCNA was detected by immunohistochemistry. The cell cycle was measured by flow cytometry. The apoptosis was detected by TIJNEL method. Results After rotavator simulating weightlessness, the cell area of SGC-7901 cells in the swivel group decreased at 12 h, 24 h, 36 h, 48 h and 72 h (P <0.01) compared with the control group. At 36 h , The length and diameter of the control group compared to the increase (P <0.01), the rest of the time no significant difference. The ratio of PCNA positive cells in SGC-7901 cells at 12 h, 24 h and 36 h was not significantly different from that in control group, and PCNA antibody positive cells in control group were significantly decreased at 48 h and 72 h (P <0.05 or P <0.01). The cell cycle of SGC-7901 cells in 12 h, 24 h and 36 h rotation groups was not significantly different from that in control group. The G0 + G1 phase cells were significantly increased after 48 h and 72 h rotation (P <0.01 or P <0 .05), while cells in S + G2 + M phase decreased significantly (P <0.01 or P <0.05). The apoptotic ratio of SGC-7901 cells in 12h, 24h, 36h and 48h rotation group was not significantly different from that in control group, and the apoptosis ratio of SGC-7901 group was higher than that of control group at 72h (P <0.01). Conclusion The morphological changes of gastric cancer SGC-7901 cells were simulated by weight loss of rotator. Cell cycle arrest and proliferation were inhibited at 48 h and 72 h, and apoptosis increased at 72 h.