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目的分析不同检测方法在梅毒血清学检测中的应用效果。方法选取苏仙区疾病预防控制中心2013—2015年收集的梅毒患者血液标本76份,分别采取梅毒螺旋体荧光抗体吸收试验(FTA-ABS)、胶体金快速检测试验(SYP)、酶联免疫吸附试验(ELISA)、梅毒螺旋体明胶凝集试验(TPPA)、梅毒螺旋体凝集试验(TPHA)、甲苯胺红不加热血清反应素试验(TRUST)进行梅毒血清学检验,并对检验结果进行对比分析。结果 FTA-ABS、ELISA、TPPA、TPHA检测各期梅毒和隐性梅毒的检出率均为100%。TRUST对一、三期梅毒及隐性梅毒检验阳性率均较SYP检查低;SYP对一期梅毒检验阳性率较ELISA、TPHA、TPPA检查低。结论 ELISA、TPHA、TPPA检查在梅毒血清检测中效果较理想,值得关注并推广。
Objective To analyze the application of different detection methods in serological detection of syphilis. Methods Seventy-six blood samples of syphilis patients were collected from Suxian Center for Disease Control and Prevention from 2013 to 2015. FTA-ABS, SYP and enzyme-linked immunosorbent assay (ELISA), TPPA, TPHA and TRUST were performed to detect the syphilis serology, and the test results were compared. Results The detection rates of syphilis and latent syphilis by FTA-ABS, ELISA, TPPA and TPHA were all 100%. The positive rates of TRUST in one or three syphilis and latent syphilis were lower than those in SYP. The positive rate of syphilis in SYP was lower than that of ELISA, TPHA and TPPA. Conclusion The results of ELISA, TPHA and TPPA in the detection of syphilis serum are more satisfactory and worthy of attention and promotion.