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目的观察羊栖菜多糖对S-180肉瘤移植小鼠免疫功能的影响,评价其抗肿瘤作用的机制。方法采用水煮醇沉、Sevag法并结合胰酶消化去蛋白及Sephadex G200柱分离纯化获得羊栖菜多糖SFP1和SFP2,用S-180肉瘤移植小鼠作为实验模型,用羊栖菜多糖SFP2腹腔注射给药,观察羊栖菜多糖对荷瘤小鼠免疫功能的调节作用。结果羊栖菜多糖能明显抑制S-180肉瘤在荷瘤小鼠上的生长,显著提高S-180荷瘤小鼠胸腺指数、脾指数及ConA诱导的脾淋巴细胞增殖,提高荷瘤小鼠NK细胞活性及腹腔巨噬细胞的吞噬活性。结论羊栖菜多糖能明显提高荷瘤小鼠机体的免疫功能,提高机体免疫功能是羊栖菜多糖抑制肿瘤生长的机制之一。
Objective To observe the effects of Sargassum polysaccharides on the immune function of mice transplanted with S-180 sarcoma and evaluate the mechanism of its anti-tumor effect. Methods The SFF1 and SFP2 polysaccharides were purified by derivatization and Sephag digestion with Sephag digestion and Sephadex G200 column. The mice were transplanted with S-180 sarcoma as an experimental model. Injected to observe the regulatory effect of Sargassum polysaccharide on immune function in tumor-bearing mice. Results Sargassum polysaccharide significantly inhibited the growth of S-180 sarcoma in tumor-bearing mice, and significantly increased thymus index, spleen index, and ConA-induced proliferation of spleen lymphocytes in S-180 tumor-bearing mice, and increased tumor-bearing mice NK Cell viability and phagocytic activity of peritoneal macrophages. Conclusion Sargassum polysaccharide can obviously improve the immune function of tumor-bearing mice, and improving immune function is one of the mechanisms of inhibiting tumor growth of Sargassum.