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目的:本研究通过比较不同药物干预下对GABAARα1、NMDAR1表达的影响,探讨柴贝止痫汤抑制颞叶癫痫发作的分子病理机制。方法:腹腔注射氯化锂-匹罗卡品制成颞叶癫痫模型,通过免疫组织化学方法和显微图像分析技术检测大鼠颞叶、海马的γ-氨基丁酸受体(GABAARα1)和谷氨酸受体(NMDAR1)的表达。结果:GABA A Rα1的表达,与空白模型组比较,柴贝止痫汤组、柴贝止痫汤联合卡马西平组癫痫大鼠海马CA1、CA3区及颞叶皮层的GABA A Rα1亚单位表达均明显增强,有高度显著性差异(P<0.01),但柴贝止痫汤组与柴贝止痫汤联合卡马西平组之间无显著性差异(P>0.05)。与正常组比较,空白模型组和卡马西平组癫痫大鼠海马CA1、CA3区及颞叶皮层的GABA A Rα1表达均明显减少,有高度显著性差异(P<0.01)。NMDAR1的表达,与正常组比较,柴贝止痫汤组、柴贝止痫汤联合卡马西平组、卡马西平组、空白模型组海马CA1、CA3区及颞叶皮层的NMDAR1表达均明显增高,有显著性差异(P<0.05),但柴贝止痫汤组、柴贝止痫汤联合卡马西平组、卡马西平组、空白模型组各组之间无显著性差异(P>0.05)。结论:柴贝止痫汤可以提高GABA A Rα1的表达,而对NMDAR1无明显影响。对GABA A Rα1表达的调节是柴贝止痫汤控制癫痫的可能机理之一。
Objective: In this study, we compared the effects of different drugs on the expression of GABAARα1 and NMDAR1, and explored the molecular mechanism of Chaibeixishi epileptic soup inhibiting temporal lobe epileptic seizures. Methods: Temporal lobe epilepsy model was made by intraperitoneal injection of lithium chloride-pilocarpine. GABAARα1 and GABA mRNAs in the temporal lobe and hippocampus of rats were detected by immunohistochemistry and microscopic image analysis. Amino acid receptor (NMDAR1) expression. Results: Compared with the blank control group, expression of GABA A Rα1 subunit of hippocampal CA1, CA3 region and temporal cortex in the Chaibeixizixian Decoction group, Chai Ba Zhixian Decoction combined with carbamazepine group (P <0.01). However, there was no significant difference (P> 0.05) between Chaibeixizhiqian Decoction group and Chai Ba Zhi Xi Tang combined with carbamazepine group. Compared with the normal group, the expression of GABA A Rα1 in hippocampal CA1, CA3 area and temporal cortex of blank model group and carbamazepine group was significantly decreased (P <0.01). NMDAR1 expression in hippocampal CA1, CA3 area and temporal cortex hippocampus were significantly higher than those in normal group (P <0.05). However, there was no significant difference between the two groups (P> 0.05), but there was no significant difference between the two groups (P> 0.05) ). Conclusion: Chaibai Zhixian Decoction can increase the expression of GABA A Rα1, but has no obvious effect on NMDAR1. The regulation of GABA A Rα1 expression is one of the possible mechanisms by which Chaibai Ehuang Decoction can control epilepsy.