目的利用模拟煤矿井下事故断食后大鼠模型探讨造成空间记忆与行为功能下降的时间性。方法选用华北理工大学动物实验中心提供的清洁级成年雄12 W龄健康SD大鼠68只,体重(350±30)g,实验前置(25±2)℃室温,自由饮食,分笼适应性喂养一周后随机分为3组:A组(n=4)为正常饮食对照;B组(n=32)只供水,不供食。C组(n=32)用于Morris水迷宫实验。B、C组又依时序性3d、5d、7d、9d各分为4个亚组(每组n=8),均单独分笼喂养。每组大鼠除进行一般行为观察及体重测量外,用Morris水迷宫系统进行PNT、SPT实验。结果 1断食早期,即3d和5d组大鼠的一般状态良好,体重虽有一定下降,但与正常组对照无统计学意义(P>0.05),PNT潜伏期缩短,表明学习记忆能力还有所增强。2断食晚期,即7d和9d组大鼠一般状况日趋变差。体重也逐渐明显下降,MWM实验中PNT潜伏期延长、PNT上台前路程显著延长、PNT实验轨迹图显示对平台的定位不够准确以及SPT平台穿越次数明显减少、SPT平台停留时间百分比明显减少、SPT实验轨迹图显示在原平台所在象限停留则相对较少,穿越原平台所在位置的次数也较少等,充分说明断食7d后空间记忆与行为能力日趋明显下降。结论完全断食后3d、5d内不会引起空间记忆与行为能力障碍,且可能由于应激的作用,在3d时学习记忆能力还稍有增强。7d后会越来越下降,直到昏迷、死亡。而完全断食的后期(7d和9d组),随着饥饿时间的延长,学习记忆功能会越来越显著下降,直到昏迷、死亡。是否存在长时间饥饿与创伤应激障碍导致应激性损伤尚有待于深入研究。 OBJECTIVE: To explore the temporal model of spatial memory and behavioral decline caused by simulating the rat model of down-time after a coal mine accident. Methods Sixty healthy adult male 12-w-age male SD rats were randomly divided into three groups: body weight (350 ± 30) g, room temperature at 25 ± 2 ℃, free diet, One week after feeding, they were randomly divided into 3 groups: group A (n = 4) served as normal diet control; group B (n = 32) was fed with water only. Group C (n = 32) was used in the Morris water maze test. Groups B and C were subdivided into 4 subgroups (n = 8 in each group) according to the temporal order of 3d, 5d, 7d and 9d. In addition to the general behavior observation and body weight measurement, each group of rats was subjected to PNT and SPT experiments using the Morris water maze system. Results In the early period of fasting, the rats in the 3d and 5d groups were generally in good condition with a slight decrease in body weight, but there was no significant difference compared with the normal control group (P> 0.05). The PNT latency was shortened, indicating that learning and memory abilities were enhanced . 2 At the end of fasting, the general condition of rats in 7d and 9d groups became worse. The weight of the MWT decreased gradually. The latency of PNT in MWM was prolonged and the length of PNT was significantly prolonged. The trajectory of PNT showed that the positioning of the platform was not accurate enough and the number of SPT platform traversal decreased significantly. The percentage of stay time of SPT platform decreased significantly. The trajectory diagram shows that there are relatively few stays in the quadrant where the original platform resides and fewer passes through the original platform, which fully shows that the spatial memory and behavioral abilities decrease significantly after 7 days of eating. Conclusions 3d and 5d after complete fasting, it does not cause spatial memory and behavioral disturbances, and learning and memory abilities may be slightly enhanced in 3d due to stress. After 7d will fall more and more, until the coma, death. However, in the later period of complete food intake (group 7d and 9d), with the prolongation of starvation, the learning and memory functions decreased more and more significantly until the coma died. Whether long-term hunger and traumatic stress disorder lead to stress damage remains to be further studied.