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研究氧化应激条件下,血氧饱和度(SO2)对红细胞氧化损伤的影响。体外将红细胞孵育至SO2分别为0.3、0.5、0.7、0.9及0.98等状态,加入终浓度为0.15mmol/L叔丁基过氧化氢(BHP)使其发生氧化应激反应。测量胞内还原性谷胱甘肽(GSH)含量反映细胞抗氧化力,以胞内高铁血红蛋白(MetHb)、膜质过氧化(TBARS)及膜上沉淀的变性珠蛋白链水平体现细胞氧化损伤程度。结果显示,BHP存在的条件下,GSH含量随SO2上升而增加;MetHb、TBARS及膜上珠蛋白链水平则随SO2的上升而下降。上述结果表明,氧化应激条件下,红细胞抗氧化力和氧化损伤与SO2密切相关,SO2的降低可促进红细胞氧化损伤。
To investigate the effect of oxygen saturation (SO2) on red cell oxidative damage under oxidative stress. In vitro, erythrocytes were incubated until SO2 was 0.3, 0.5, 0.7, 0.9 and 0.98, respectively. Oxidative stress was induced by adding 0.15 mmol / L t-butyl hydroperoxide (BHP). The content of intracellular reductive glutathione (GSH) was measured to reflect the antioxidant capacity of cells. The level of cellular oxidative damage was measured by intracellular methemoglobin (MetHb), membrane lipid peroxidation (TBARS) and denatured globin chain, . The results showed that the content of GSH increased with the increase of SO2 in the presence of BHP while the levels of MetHb, TBARS and membrane globin chain decreased with the rise of SO2. The above results show that under oxidative stress, antioxidant activity and oxidative damage of erythrocytes are closely related to SO2, and SO2 reduction can promote oxidative damage of erythrocytes.