蜜环菌是天麻种植的共生菌,传统培养方式存在试管斜面培养菌种量小、PDA培养基种性易退化、固体培养生长不理想以及试管表面接种易污染等问题。通过改试管斜面为试管菌柱、固体方式为半固体方式、PDA培养基为综合培养基、试管表面转扩为试管底部破碎转扩等蜜环菌母种培养与转扩方式的改革,提高了蜜环菌母种的制作质量和转扩成功率。 Armillaria mellea is a symbiotic bacteria cultivated by Gastrodia elata Blume. There are some problems in traditional culture such as the small amount of culture tube in bevel, the easy degeneration of PDA medium, the poor growth of solid culture and the easy inoculation of tube surface. By changing the slope of the test tube into a test tube fungus column, the solid mode is a semi-solid mode, the PDA medium is a comprehensive medium, and the surface of the test tube is expanded and expanded to transform the propagation and propagation mode of Armillaria mellea in the bottom of the test tube. Meloidogyne mother of the production quality and success rate of expansion.