论文部分内容阅读
目的：探讨纤维蛋白单体引起细胞伸展的机理。方法：通过２ｍｏｌ／ＬＮａＢｒ溶解纤维蛋白制成纤维蛋白单体。采用细胞粘附及抑制方法观察细胞伸展状况。结果：人神经胶质瘤细胞不能在固定的纤维蛋白原上伸展，但能在纤维蛋白单体上伸展，此伸展过程不能被外来的纤维蛋白原、片段Ｅ和Ｄ抑制，但能被纤维蛋白单体和ＲＧＤ（精氨酸－甘氨酸－天冬氨酸）序列所抑制。结论：纤维蛋白单体引起细胞伸展的可能机理为纤维蛋白单体α链上的两个ＲＧＤ序列Ａα（９５－９７）和Ａα（５７２－５７４）暴露，从而促使纤维蛋白单体与细胞作用，调节细胞伸展
Objective: To investigate the mechanism of fibrin monomer causing cell expansion. Methods: Fibrin monomers were prepared by dissolving fibrin with 2 mol / L NaBr. Cell adhesion and inhibition methods were used to observe cell expansion. Results: Human glioma cells can not extend on fixed fibrinogen but can extend on fibrin monomers and this stretching process can not be inhibited by extraneous fibrinogen, fragments E and D but can be inhibited by fibrin Monomeric and RGD (arginine-glycine-aspartate) sequences. CONCLUSION: The possible mechanism by which fibrin monomers cause cell elongation is the exposure of two RGD sequences Aα (95-97) and Aα (572-574) on the α-chain of fibrin monomer to promote the action of fibrin monomers and cells. Regulate cell stretch