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目的：探讨单纯疱疹病毒胸苷激酶（HXV－TK）基因在人膀胱癌基因治疗中的作用。材料与方法：利用电击基因导入法，将逆转录病毒载体PLNXXTK导入包装细胞PA317，经G418筛选，获得稳定产病毒的PA317／PLNXXTK（PA317／TK）细胞株。采用制备的病毒液转染体外培养的人膀胱癌T24细胞，并联合丙氧鸟苷（GCV）作用，研究其对膀胱癌细胞的生长抑制作用。结果：所获得的PA317／TK细胞能稳定地产生复制缺陷的逆转录病毒载体，滴度为1．65×10~5 cfu／ml。结论：逆转录病毒介导的HXV－TK基因在体外能成功地转染T24细胞，且HXV－TK／GCV系统能明显抑制膀胱癌细胞的生长。这为HXV－TK／GCV在人类膀胱癌治疗方面的进一步体内实验提供了可靠依据。
Objective: To investigate the role of herpes simplex virus thymidine kinase (HXV-TK) gene in the gene therapy of human bladder cancer. MATERIALS AND METHODS: PA317 / PLNXXTK (PA317 / TK) cell line was obtained by electroporation gene transfer method. The retroviral vector PLNXXTK was introduced into the packaging cell line PA317 and screened by G418. The human bladder cancer T24 cells cultured in vitro were transfected with the prepared virus liquid, and combined with the effect of gonadotrophin (GCV) on the growth inhibition of bladder cancer cells. Results: The obtained PA317 / TK cells stably produced replication-defective retroviral vector with a titer of 1.65 × 10 ~ 5 cfu / ml. CONCLUSION: Retrovirus-mediated HXV-TK gene can successfully transfect T24 cells in vitro, and HXV-TK / GCV system can significantly inhibit the growth of bladder cancer cells. This provides a reliable basis for further in vivo experiments of HXV-TK / GCV in the treatment of human bladder cancer.