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目的:采用高效液相色谱法比较半夏泻心汤及其苦降药组指纹图谱的差别。方法:采用PhenomenexGemini C18 110A(5μm,250mm×4.6mm)色谱柱,柱温40℃,乙腈-0.05%三乙胺水溶液(pH 3.0)梯度洗脱,检测波长275nm,对半夏泻心汤及其苦降药组水提醇沉液的HPLC指纹图谱进行比较分析。结果:该方法精密度、稳定性和重复性良好,相似度均大于0.99,在半夏泻心汤中17个和其苦降药组共有的色谱峰峰面积占总峰面积98.18%。结论:苦降药组水提醇沉液和半夏泻心汤水提醇沉液HPLC指纹图谱有一定的差别,但差别不大。
OBJECTIVE: To compare the difference of fingerprints of Banxiaxiexin Decoction and its bitter medicine by HPLC. METHODS: Phenomenex Gemini C18 110A (5 μm, 250 mm × 4.6 mm) column was used to elute gradient elution of acetonitrile-0.05% triethylamine in water (pH 3.0) at a column temperature of 40 ℃. The detection wavelength was 275 nm. HPLC fingerprints of water-reducing and alcohol-lowering solution of bitter medicine group were comparatively analyzed. Results: The precision, stability and repeatability of this method were good. The similarities were all greater than 0.99. The peak area of the chromatographic peaks shared by 17 and its bitter herb groups in Banxiaxiexin Decoction accounted for 98.18% of the total peak area. Conclusion: There are some differences in HPLC fingerprints between water-reducing and alcohol-lowering solution of Pengyuxiao medicine group and alcohol extract of Banxiaxiexin soup, but the differences are not obvious.