[Objective] To research the inhibitory effects of wasabi extracts on the proliferation of SW480 cells and its mechanism. [Method] SW480 cells were treated by different concentrations of wasabi extracts for 48 h. Effects on the proliferation of SW480 cells were detected by MTT method; changes of the apoptosis and cell cycle of SW480 cells were detected by flow cytometry; effects of wasabi extracts on the transcription level of P16INK4a mRNA in SW480 cells were detected by RT-PCR. [Result] Wasabi extracts had inhibitory effects on the proliferation of SW480 cells in a concentration-dependent manner; and the IC50 value was about 130 μmol/L. The cell cycle was blocked at G2 stage. The transcription level of P16INK4a mRNA in SW480 cells reduced in a concentration-dependent manner. [Conclusion] Wasabi extracts had inhibitory effects on the proliferation of SW480 cells, could induce apoptosis, and blocked the cell cycle at G2 stage by restricting the transcription of P16INK4a mRNA. [Objective] To research the inhibitory effects of wasabi extracts on the proliferation of SW480 cells and its mechanism. [Method] SW480 cells were treated by different concentrations of wasabi extracts for 48 h. Effects on the proliferation of SW480 cells were detected by MTT method ; changes of the apoptosis and cell cycle of SW480 cells were detected by flow cytometry; effects of wasabi extracts on the transcription level of P16INK4a mRNA in SW480 cells were detected by RT-PCR. [Result] Wasabi extracts had inhibitory effects on the proliferation of The cell cycle was blocked at G2 stage. The transcription level of P16INK4a mRNA in a concentration-dependent manner; and the IC50 value was about 130 μmol / L. extracts had inhibitory effects on the proliferation of SW480 cells, could induce apoptosis, and blocked the cell cycle at G2 stages by restricting the transcription of P16INK4a mRNA.