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目的 :探讨uPA和uPARmRNA表达水平与胃癌细胞恶性程度、浸润转移的关系及其临床意义。方法 :运用cDNA mRNA斑点杂交技术定量、平行地检测了 2 3例胃癌及相应癌旁组织中uPA和uPARmRNA表达水平 ,并综合分析了uPA和uPARmRNA表达水平与胃癌临床生物学指征的相关意义。结果 :uPA和uPARmRNA表达水平在 2 3例胃癌组织中 ,分别有 18例uPA和 19例uPAR高于相应癌旁组织 ;14例uPA和uPAR协同高于癌旁组织 4倍以上 ;在伴有淋巴结转移和浸润到肌层、浆膜层的胃癌组织中均明显增高 ;Ⅲ、Ⅳ期胃癌组织也明显高于Ⅰ、Ⅱ期胃癌组织。结论 :在基因转录水平 ,胃癌组织中uPA和uPAR表达呈增高趋势 ;具有浸润转移的胃癌组织表达增强 ;uPA和uPAR基因的高表达是影响胃癌细胞恶性程度和浸润转移的重要因素之一。
Objective: To investigate the relationship between the expression of uPA and uPAR mRNA and the malignant degree, infiltration and metastasis of gastric cancer cells and its clinical significance. Methods: The expression levels of uPA and uPAR mRNA in 23 cases of gastric cancer and corresponding paracancerous tissues were detected quantitatively and in parallel by dot blot hybridization with cDNA. The correlation between the expression of uPA and uPAR mRNA and clinical signs of gastric cancer was analyzed. Results: The expression levels of uPA and uPAR mRNA in 23 cases of gastric cancer tissues were 18 cases of uPA and 19 cases of uPAR respectively, which were higher than that of corresponding paracancerous tissues; 14 cases of uPA and uPAR were higher than 4 times of paracancerous tissues; Metastasis and infiltration into the muscular layer, serosa in gastric cancer were significantly increased; Ⅲ, Ⅳ gastric cancer tissue was significantly higher than Ⅰ, Ⅱ gastric cancer. CONCLUSION: The expression of uPA and uPAR in gastric cancer tissue is increased at gene transcription level. The expression of uPA and uPAR gene is enhanced in gastric cancer with invasion and metastasis. The high expression of uPA and uPAR is one of the important factors that affect the malignant degree and invasion and metastasis of gastric cancer.