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目的观察不同剂量高功率微波(high power microwave,HPM)辐照对Wistar大鼠脑组织的损伤作用及氧化应激的影响,为建立微波辐射氧化损伤动物模型及抗辐射药物的评价提供依据。方法将80只健康成年雄性Wistar大鼠按析因设计随机分为16组,辐射平均功率密度分别为0、10、30及100 m W/cm2;取材时间分别为6 h、1、3及7 d。照射组照射时间为6 min。分别于各取材时间点活杀动物并取脑组织,采用比色法测量大鼠脑组织丙二醛(MDA)含量、蛋白质羰基含量和谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性。结果各辐射组MDA、蛋白质羰基含量基本随着辐射剂量的升高而升高,随取材时间的延长而降低。各辐射组SOD、GSH-px、CAT活性基本随辐射剂量的升高而降低,随取材时间的延长先降后升。结论微波辐射能引起大鼠脑组织的氧化应激反应,主要表现在大鼠脑组织脂质和蛋白质氧化损伤以及抗氧化物酶活性降低,且与辐射剂量、取材时间有一定的依赖性。
Objective To observe the effects of different doses of high power microwave (HPM) irradiation on brain injury and oxidative stress in Wistar rats, and to provide basis for establishing an animal model of oxidative damage by microwave radiation and evaluation of anti-radiation drugs. Methods Eighty healthy adult male Wistar rats were randomly divided into 16 groups according to the factorial design. The average power density of radiation was 0, 10, 30 and 100 mW / cm2, respectively. The extraction time was 6 h, 1, 3 and 7 d. Irradiation group irradiation time was 6 min. The animals were killed and the brain tissues were sacrificed at each time point. The contents of malondialdehyde (MDA), protein carbonyls and glutathione peroxidase (GSH-Px) Oxide dismutase (SOD), catalase (CAT) activity. Results The contents of MDA and protein carbonyl in radiation group increased with the increase of radiation dose and decreased with the increase of exposure time. The activity of SOD, GSH-px and CAT in each radiation group basically decreased with the increase of radiation dose, and then decreased with the extension of time. CONCLUSION: Microwave radiation can cause oxidative stress in rat brain. It is mainly manifested in oxidative damage of lipid and protein and decrease of antioxidant enzyme activity in brain tissue of rats, and it has some dependence on radiation dose and drawing time.