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目的 采用仓鼠到大鼠移植模型 ,观察移植物存活时间与受体血清IgM型诱生抗体水平的关系。 方法 仓鼠心脏移植于SD大鼠颈部 ,按不同剂量及配对方案给予免疫抑制剂 ,单磷酸肌苷 (次黄嘌呤苷 )脱氢酶抑制剂(ERL) 10～ 30mg/(kg·d)、雷帕霉素 (RAD) 1 0～ 1 5mg/(kg·d)、环孢素A (CyA) 5～ 10mg/(kg·d)。观察移植心存活时间 ,ELISA法测定移植心被排斥时血清IgM型诱生抗体水平 ,计算两者的相关系数。 结果 对照组移植心平均存活时间为 (3 7± 1 1)d。单用ERL和CyA组移植心存活时间无延长 ,RAD组有一定的延长 (6 0± 0 9)d ,P <0 0 1;联合用药组可见移植物存活时间明显延长 (P <0 0 1) :ERL +CyA、ERL +RAD、RAD +CyA及RAD +ERL +CyA组的移植物平均存活时间分别为 (8 0± 3 5 )d、 (10 3± 4 7)d、 (12 8± 2 9)d及 (10 0± 2 2 )d。排斥时大鼠抗仓鼠IgM反应显著受抑制 ,且平均抗体水平与平均移植物存活时间呈负相关 (r =- 0 86 2 ;P <0 0 5 )。结论 仓鼠移植物存活时间与受体血清IgM型诱生抗体水平呈显著的负相关 ,ERL、RAD与CyA配合使用能有效抑制大鼠抗仓鼠IgM并明显延长异种移植物的存活时间。
Objective To study the relationship between the survival time of graft and the level of IgM antibody induced by the receptor using hamster to rat transplantation model. Methods Hamster heart was transplanted into the neck of SD rats. The immunosuppressive agents, inosine monophosphate (Hyp inosine monophosphate) dehydrogenase inhibitor (ERL) 10 ~ 30mg / (kg · d) Rapamycin (RAD) 10 ~ 15mg / (kg · d), cyclosporin A (CyA) 5 ~ 10mg / (kg · d). The survival time of transplanted heart was observed. The level of IgM antibody induced by graft rejection was measured by ELISA, and the correlation coefficient was calculated. Results The mean survival time of the control group was (37 ± 11) days. There was no prolongation of survival time after transplanted with ERL and CyA alone, and the prolongation of RAD group was prolonged (60 ± 0 9) days, P <0 01. The graft survival time was significantly prolonged in combination group (P <0.01) ): The average graft survival time of ERL + CyA, ERL + RAD, RAD + CyA and RAD + ERL + CyA group were (80 ± 3 5) d, (10 3 ± 4 7) d, (12 8 ± 2 9) d and (10 0 ± 2 2) d. The rejection of anti-hamster IgM in rats was significantly inhibited at rejection, and the mean antibody level was negatively correlated with mean graft survival (r = - 0 862; P <0 05). Conclusion The survival time of Hamster grafts is significantly negatively correlated with the antibody level of IgM-inducing antibody. The combination of ERL, RAD and CyA can effectively inhibit the anti-hamster IgM in rats and prolong the survival time of xenografts.