论文部分内容阅读
目的采用絮凝技术优化消炎退热颗粒原药水提液除杂净化工艺,保留有效成分的同时去除更多杂质,提高原药水提液的澄清度。方法明确消炎退热颗粒原药水提液中杂质的成分后,通过筛选絮凝剂的实验,确定了采用壳聚糖絮凝剂对原药水提液进行絮凝除杂。针对以消炎退热颗粒原药水提液中有效成分秦皮乙素的保留率,杂质成分蛋白质和鞣质的去除率以及絮凝处理后药液上清液的浊度作为研究指标,分别研究考察了壳聚糖絮凝剂的配比及用量、絮凝温度、絮凝快搅速度及快搅时间对絮凝整体效果的影响。结果通过实验得出壳聚糖作为絮凝剂用于消炎退热颗粒原药水提液除杂净化的最佳絮凝工艺条件为壳聚糖加入量1.25 g/L,絮凝温度为40℃,快搅速度为350 r/min,快搅时间为3 min。该条件下有效成分秦皮乙素的保留率为88.92%,蛋白质和鞣质的去除率分别为62.96%和67.19%,絮凝后药液静置24 h后浊度为5.0 NTU。结论壳聚糖作为絮凝剂用于消炎退热颗粒原药水提液的除杂净化,能有效提高药液中杂质的去除率,同时能提高药液的澄清度。
OBJECTIVE To optimize the decontamination and purification process of water extract of anti-inflammatory and antipyretic granules by flocculation technology, and to remove more impurities while retaining the active ingredients and improve the clarity of the original medicinal liquid extract. Methods After clarifying the components of the impurities in the anti-inflammatory and antipyretic prodrugs, the flocculation and impurity removal of the original medicinal solution was confirmed by the experiment of screening flocculant. Aiming at the retention rate of aescin, the removal of protein and tannin, and the turbidity of liquid supernatant after flocculation treatment, the effects of the contents of aesculetin, Proportion and amount of flocculant, flocculation temperature, flocculation fast stirring speed and fast stirring time on the overall flocculation effect. Results The best flocculation conditions of Chitosan as flocculant for the anti-inflammatory and antipyretic granules were as follows: chitosan dosage 1.25 g / L, flocculation temperature 40 ℃, fast stirring speed 350 r / min, fast stirring time of 3 min. Under these conditions, the retention rate of aescine was 88.92%, the removal rates of protein and tannin were 62.96% and 67.19%, respectively. The turbidity of the solution was 5.0 NTU after standing for 24 h. Conclusion Chitosan as flocculant can be used to purify the anti-inflammatory and antipyretic granules. The removal efficiency of impurities in the anti-inflammatory and antipyretic granules can be effectively improved, and the clarity of the liquid can be improved at the same time.