采用(S)-2-氨甲基四氢吡咯作为手性单体,以键合在3-氨丙基硅胶上的4,4’-偶氮-二(4-氰基)戊酸(ACVA)作为引发剂,通过直接引发表面聚合反应,制备了一种新型HPLC刷型手性固定相CSP-a。采用乙腈-甲醇为流动相,在柱温30℃和UV 254 nm检测条件下实现了对联萘酚衍生物、氨基酸衍生物的色谱拆分。结果表明,CSP-a对π酸和π碱类化合物都有明显拆分效果。 Using (S) -2-aminomethyltetrahydropyrrole as a chiral monomer, 4,4’-azo-bis (4-cyano) pentanoic acid (ACVA ) As an initiator, a novel HPLC brush-type chiral stationary phase CSP-a was prepared by directly initiating surface polymerization. Using acetonitrile-methanol as the mobile phase, the chromatographic resolution of the derivatives and amino acid derivatives of binaphthol was achieved under the conditions of 30 ℃ and UV detection at 254 nm. The results showed that CSP-a had obvious resolution effects on both π-acids and π-bases.