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研究黄芪与红芪药材之间的DNA分子鉴别方法。采集不同产地的黄芪药材30份、红芪28份,所有样品进行总DNA的提取,通过对黄芪及红芪药材trnL-trnF片段进行扩增、测序,进行同源比对后根据其变异位点设计特异性鉴别引物。并对位点特异性PCR方法进行条件优化。通过使用特异性引物对所有样品进行PCR扩增,发现黄芪可扩增出136 bp片段,红芪可以扩增出323 bp片段。黄芪中掺入10%以上红芪可以扩增出红芪特异鉴别条带。通过位点特异性PCR的方法实现了黄芪与红芪药材的快速、准确鉴别。
To study the molecular identification of DNA between Astragalus and Radix Hedysari. 30 samples of Radix Astragali collected from different areas, 28 parts of Radix Hedysari, total DNA were extracted from all the samples. The trnL-trnF fragments of Radix Astragali and Radix Hedysari were amplified and sequenced, Design specific identification primers. Site-specific PCR was optimized. Through the use of specific primers for all samples PCR amplification, found that Astragalus can amplify a 136 bp fragment, Radix can amplify a 323 bp fragment. Astragalus mixed with Radix Astragali 10% or more can be differentiated to identify syringomyelia band. Through the method of site-specific PCR, the rapid and accurate identification of Radix Astragali and Radix Astragali was achieved.