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采用 NBT 光化还原抑制技术检测8个草菇菌株的6个不同周(?)期的菌丝体超氧化物歧化酶(SOD)活性水平发现,草菇菌丝体在第2周龄期 SOD 活性最强高的可达光化还原抑制47%。低的为28%。从第3周龄期至第6周龄期 SOD 活性反复波动,(?)均在第2周龄期活力水平之下。菌丝 SOD 活性水平,可反映出草菇菌株子实体产量和生长速度水平。从不同龄期菌丝产量变化表明菌丝生长快、衰老快和自溶快等特性。采用聚丙烯酰胺凝胶电泳结果表明,参试的8个菌株6个周龄期的草菇菌丝在凝胶板上 Rf 值0.3附近有一条 SOD 同工酶强带,这条带可能属于 Mn-SOD 类型。从菌丝体 SOD 同工酶谱情况,可判断草菇子实体(?)伞的快慢。
NBT photoreduction inhibition technique was used to detect the level of superoxide dismutase (SOD) activity in mycelia of 8 strains of mushroom at 6 different weeks. The most potent up to 47% inhibition of photoreduction. The low is 28%. The SOD activity fluctuated repeatedly from the 3rd week to the 6th week, and (?) Were below the level of the 2nd week. Mycelium SOD activity level, which can reflect the fruiting body fruiting bodies of mushroom yield and growth rate. The changes of mycelium yield from different ages showed that the mycelium grows fast, senescence fast and autolysis characteristics. The result of polyacrylamide gel electrophoresis showed that there were one strong band of SOD isozymes in the 6 weeks-old mushroom strains of mycorrhizal fungi on the gel plate near the Rf value of 0.3, which belonged to Mn -SOD type. From the mycelium SOD isozyme profile, can determine the fruiting bodies (?) Umbrella speed.