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为建立快速检测蔬菜、茶叶中克百威残留的酶联免疫方法,合成克百威半抗原,并与载体蛋白偶联合成人工抗原,然后免疫制备其单克隆抗体,在筛选克百威单克隆抗体的基础上,建立酶联免疫检测方法。结果表明,Logit/Log拟合标准曲线为y=-2.054x+0.667 3,相关系数(r)为0.998 5,半数抑制浓度(IC_(50))为2.3μg/L,对蔬菜、茶叶样本的检测限分别为10、5μg/kg,加标回收率为79.2%~102.7%,样本重复检测的变异系数为5.5%~11.5%。结果表明,建立的酶联免疫检测方法具有较好的特异性、准确性和重复稳定性,可用于蔬菜、茶叶中克百威残留的检测。
In order to establish a rapid enzyme-linked immunosorbent assay (ELISA) for the determination of carbofuran in vegetables and tea, synthesize carbofuran hapten and couple it with carrier protein to synthesize artificial antigen, then immunize to prepare its monoclonal antibody. Antibodies based on the establishment of enzyme-linked immunosorbent assay. The results showed that the Logit / Log fitting standard curve was y = -2.054x + 0.667 3, the correlation coefficient (r) was 0.998 5, the half inhibitory concentration (IC 50) was 2.3 μg / L, The detection limits were 10, 5μg / kg respectively. The spiked recoveries ranged from 79.2% to 102.7%. The coefficient of variation (CV) was 5.5% ~ 11.5%. The results showed that the established ELISA method has good specificity, accuracy and repeatability and can be used for the determination of carbofuran residue in vegetables and tea.