水泥基材料内部水分的传输与分布对材料性能和耐久性有重要意义.传统的宏观土木工程监测湿度设备并不能原位捕捉水泥基材料内部微细观尺度的湿度情况.本文提出一种利用罗丹明6G荧光表征不饱和水泥基材料内部相对湿度变化的方法,设计了预先拌入式和成型后涂抹式两种成型工艺,探索了最佳配制浓度.试验结果表明:罗丹明6G可以通过荧光强度原位反映水泥基材料薄层的相对湿度,对水泥基材料水化放热和水化产物的几乎没有影响;荧光强度在内部相对湿度为63％～75％时较高.研究结果为生物荧光材料在建筑外墙容易因湿度累积导致空鼓脱落、结构混凝土表层易开裂损伤区域的监测、检测提供了基础研究.“,”The transmission and distribution of moisture in cement-based materials are of great significance to the properties and durability of materials. Traditional macro-humidity monitoring equipment in civil engineering cannot capture the microscale humidity inside cement-based materials in situ. In this paper, a method of using rhodamine 6G fluorescence to characterize the change in relative humidity in cement-based materials is proposed. Two kinds of moulding processes are designed, which are premixed and smeared after moulding, and the optimal preparation concentration is explored. The results showed that rhodamine 6G can reflect the relative humidity of cement-based materials in situ by its fluorescence intensity and had little effect on the hydration heat release and hydration products of cement-based materials; the fluorescence intensity was much higher when the internal relative humidity was 63％ and 75％. The research results lead the application of polymer materials in the field of traditional building materials, help to explore the performance evolution law of cement-based materials in micro scale, and have important significance for the evolution from single discipline to interdisciplinary.