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应用聚合酶链反应（ＰＣＲ）和酶联免疫吸附试验（ＥＬＩＳＡ）动态检测了中老年乙型肝炎病人不同时期的血清标志。结果表明，ＨＢｅＡｇ阳性的血清其ＰＣＲ检测ＨＢＶＤＮＡ均阳性，两者呈平行关系，１～４ｗ后有２０％病人血清ＨＢＶＤＮＡ转阴，同时伴有部分病人ＨＢｅＡｇ消失或ＨＢｅＡｂ出现，其他ＨＢＶ血清学标志物阳性的病人血清ＨＢＶＤＮＡ阳性率明显低于ＨＢｅＡｇ阳性血清，且ＨＢＶＤＮＡ暂转阴率高于ＨＢｅＡｇ阳性血清，ＨＢＶＤＮＡ转阴的时间也短（１～４ｗ）。动态检测ＨＢＶＤＮＡ可以动态观察ＨＢＶＤＡＮ的复制，对指导临床治疗具有很大的意义。
Polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) were used to detect serum markers in different stages of middle-aged and elderly patients with hepatitis B virus. The results showed that HBeAg positive serum PCR detection of HBVDNA were positive, the two parallel relationship between 1 ~ 4w after 20% of serum HBVDNA negative patients, accompanied by some patients with HBeAg disappearance or HBeAb, other HBV serological markers Positive patients serum HBVDNA positive rate was significantly lower than HBeAg positive serum, and HBVDNA transient negative rate higher than HBeAg positive serum, HBVDNA negative time is also short (1 ~ 4w). Dynamic detection of HBVDNA can dynamically observe the replication of HBVDAN, to guide the clinical treatment of great significance.