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Objective: The aim of the study was to establish models of Hep-2 laryngeal cancer cell line of different oxygen supplying,trying to investigate the impact of normoxia,hypoxia,reoxygenation after hypoxia on apoptosis and expression of proteins HIF-1α and p53 to Hep-2 human laryngeal cancer cell line induced by 60Co Y-ray.Methods: Human laryngeal cancer Hep-2 cells were divided into 3 groups: group A(normoxia),group B(hypoxia),and group C(reoxygenation after hypoxia).All of the cells were exposed to 5 Gy dosage of y-ray.Flow cytometry(FCM)was used to measure the protein levels of HIF-1αand p53 and to detect cell apoptosis.The protein levels of HIF-lo and p53 were also determined by immunohistochemistry and Western blotting.The expression of HIF-1a mRNA was determined by RT-PCR.Results: The protein levels of HIF-1a and p53 were evidently increased in group B compared to group A.The protein levels of HIF-1α and p53 in group C were lower compared to group B; the rate of apoptosis in group C was higher than that in group B.Conclusion: Hypoxia decreased the effect of apoptosis induced by 60Co Y-ray in Hep-2 human laryngeal cancer call line.The apoptosis pathway maybe related to some other genes or proteins but not p53 in the conditions of hypoxia and reoxygenation after hypoxia.