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目的 为了研究核转录因子 (NF κB)在老化小鼠各脏器中的表达及其活性的改变 ,以期探讨其在老化及多器官功能不全发生过程中的作用。方法 用免疫组化法检测NF κB在小鼠各脏器中的表达情况 ,同时用凝胶阻滞分析法观察NF κB的活性。结果 在老化小鼠的各脏器中 ,NF κB的表达量及活性均有不同程度的增加 ,尤以肺脏的改变最为显著。结论 NF κB可能是老化及肺启动的老年多器官功能不全过程中的一个非常关键的转录因子。
Objective To investigate the expression of nuclear factor kappa B (NFκB) in various organs of aged mice and the changes of its activity in order to explore its role in the process of aging and multiple organ dysfunction. Methods The expression of NF-κB in various organs of mice was detected by immunohistochemistry, and the activity of NF-κB was detected by gel block assay. Results The expression and activity of NF-κB in various organs of aging mice all increased to some extent, especially in the lungs. Conclusions NF κB may be a very critical transcription factor in aging and lung-induced senile multiple organ dysfunction.