论文部分内容阅读
从单个杜氏利什曼和热带利什曼前鞭毛体分离克隆发展了用半固体琼脂的一种简易的平皿法。两种原虫在体外的繁殖用Dulbecco’s改良的Eagle组织培养基,增加碳酸氢纳3.7g/L,氯化血红素5mg/L,嘌呤源(Purine Source)0.1mM,和未透析的胎牛血清(10%)的半规定培养基,或用0.3%牛血清白蛋白加吐温代替胎牛血清的全规定培养基,能形成高能的离散群体。杜氏利什曼原虫形成肉眼可见的群落时间是7～9天,热带利什曼原虫出现明显的群落是8～14天。使用全规定培养基形成群落的时间为50%,但不影响克隆化的效率。作者指出:在完全培
Cloning from individual Duchenne Leishmania and Leishmania promastigotes developed a simple platelet method using semi-solid agar. In Vitro Propagation of Two Protozoa Using Dulbecco’s Modified Eagle’s Tissue Culture Medium, 3.7 g / L sodium bicarbonate, 5 mg / L hemin, 0.1 mM Purine Source, and undiluted fetal bovine serum 10%) of the semi-defined medium, or 0.3% bovine serum albumin plus tween instead of fetal bovine serum in the entire medium, to form a high-energy discrete population. Leishmania donovani form visible community time is 7 to 9 days, tropical Leishmania obvious community is 8 to 14 days. The time required to form a community using the fully defined media was 50%, but did not affect the efficiency of cloning. The author states: In complete training