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目的:对《中国药典》中桑叶含量测定项下方法进行改进。方法:采用HPLC法,Agilent Zorbax SB-C18色谱柱(250mm×4.6 mm,5μm);流动相为乙腈-0.2%磷酸水溶液(梯度洗脱),流速为1.0 ml·min~-1;检测波长为354 nm;柱温为30℃。结果:《中国药典》2010年版桑叶含量测定项下测得的芦丁含量实为芦丁和异槲皮苷的含量之和,优化后方法可分离该两种化合物,且芦丁和异槲皮苷分别在2.76~27.60μg·ml-1(r=0.999 9)和4.74~47.39μg·ml-1(r=0.999 8)范围内与峰面积呈良好的线性关系;平均加样回收率分别为100.31%(RSD=0.83%)和100.32%(RSD=1.04%)(n=6)。结论:优化后方法简便、稳定、重复性好,可用于桑叶的质量控制。
Objective: To improve the method of determination of mulberry leaf content in Chinese Pharmacopoeia. Methods: An Agilent Zorbax SB-C18 column (250 mm × 4.6 mm, 5 μm) was used with a mobile phase of acetonitrile-0.2% phosphoric acid (gradient elution) at a flow rate of 1.0 ml · min -1. The detection wavelength was 354 nm; column temperature was 30 ℃. Results: The content of rutin measured under the Chinese Pharmacopoeia 2010 edition of mulberry leaf content was actually the sum of rutin and isoquercitrin. The optimized method could separate the two compounds, and rutin and isoquercitrin The glycosides showed a good linear relationship with the peak area in the range of 2.76-27.60μg · ml-1 (r = 0.999 9) and 4.74-47.39μg · ml-1 (r = 0.999 8), respectively. The average recoveries were 100.31% (RSD = 0.83%) and 100.32% (RSD = 1.04%) (n = 6). Conclusion: The optimized method is simple, stable, reproducible and can be used for the quality control of mulberry leaves.