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柳树耐盐亲本“9901”和敏盐亲本“沿江柳”进行室内人工杂交,构建柳树的耐盐分离群体。首先通过流式细胞仪测定亲本与杂交F_1代的染色体倍性,再通过对亲本和F_1代的DNA提取进行测序和技术分析鉴定亲子异常,在此基础上,通过不同NaCl浓度的水培柳树杂交F_1代扦插枝条快速进行耐盐性鉴定。结果表明:亲本及杂交F_1代染色体均属于四倍体,随机选取的200个杂交F_1代中有195个鉴定为真杂种,经耐盐性测定存在明显的分离现象,成功地构建了柳树的耐盐性分离群体。为柳树耐盐性的遗传分析、遗传图谱的构建和耐盐性遗传QTL定位提供了一定的理论参考依据。
Willow salt-tolerant parents “9901 ” and sensitive salt parent “willow along ” artificial hybridization to build the willow salt tolerance population. Firstly, the chromosome ploidy of F_1 generation was detected by flow cytometry, and then the parental and F_1 generation DNAs were sequenced and analyzed by sequencing to identify parental abnormalities. On the basis of this, F_1 generation of cuttings quickly salt tolerance identification. The results showed that all F1 progenies and F1 hybrids were tetraploid. 195 out of 200 randomly selected F1 progenies were identified as true hybrids. Significant segregation was observed in salt tolerance and the tolerance of Willow Salt separation groups. It provided some theoretical references for genetic analysis of salt tolerance of willow, construction of genetic map and genetic QTL for salt tolerance.