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以两个杂交组合（组合Ⅰ：吉林28×吉林27；组合Ⅱ：吉林26×吉林20）的F1与其亲本分别回交，形成B1F1、B2F1世代，自交加代形成B1F2和B2F2及B1F3和B2F3世代，分析了高蛋白和高产亲本回文对后代蛋白质含量及籽粒产量的影响。结果表明，回交后代BF1和BF2平均蛋白质含量及BF2变异幅度随回交亲本蛋白质含量的高低而变化。BF2蛋白质含量总变异幅度略小于F2，并随回交亲本蛋白质含量的高低，表现为偏态分布。与低蛋白高产亲本回交，BF2群体中低蛋白个体的比例增加，小于或等于低值亲本的比例两组合分别为9．2％和16．4％；相反，以高蛋白品种作回交亲本的BF2世代，高蛋白个体的比例提高，大于或等于高值亲本的比例，两组合分别达50．4％和36．4％。回交后代BF2、BF3平均产量与亲本产量水平方差分析结果呈现与蛋白质含量变化的相反趋势。
F1 hybrids with F1 hybrids (combination Ⅰ: Jilin 28 × Jilin 27; combination Ⅱ: Jilin 26 × Jilin 20) were backcrossed with their parents to form B1F1 and B2F1 generations, selfing to form B1F2 and B2F2 and B1F3 and B2F3 generations respectively The effects of high-protein and high-yielding parents on the protein content and grain yield of offspring were analyzed. The results showed that the average protein content of BF1 and BF2 and the amplitude of BF2 variation varied with the level of backcross parental protein content. The total variation of BF2 protein content was slightly less than F2, and showed a skewed distribution with the protein content of the backcross. Backcrossing with low-protein and high-yielding parents, the proportion of low-protein individuals in the BF2 population increased by 9.2% and 16.4%, respectively, for the less than or equal to the low-value parent. On the contrary, BF2 generation, the proportion of high-protein individuals increased, greater than or equal to the proportion of high-value parents, the two combinations were 50.4% and 36.4%. The results of variance analysis of BF2, BF3 average yield and parental yield showed the opposite trend with the changes of protein content.