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目的了解本地区女性生殖道HPV感染情况和基因分型的分布特征,为本地区女性HPV的感染提供流行病学依据。方法采用流式荧光杂交法对9 177例女性生殖道标本进行HPV DNA分型检测,并对HPV总感染率、亚型分布特征、重叠感染率、年龄差异进行统计分析。结果本地区女性HPV检出率为16.33%,高危型HPV的检出率为11.09%,易感高危亚型为HPV53(1.37%)、HPV16(1.35%)和HPV52(1.31%);低危型HPV的检出率为5.24%,易感低危亚型为HPV61(1.01%)和HPV43(0.99%)。≤20岁、51岁~和≥61岁的女性HPV阳性率较高,且≤20岁的女性以双重感染为主,占45.83%,其余各年龄组女性以单一感染为主。≤20岁的女性HPV亚型检出率最高。结论流式荧光杂交技术可用于HPV DNA的分型检测,为本地区女性HPV疫苗接种和宫颈癌防治工作提供一定的参考依据。
Objective To understand the distribution of HPV infection and genotyping in the female genital tract in this area and to provide epidemiological basis for the HPV infection in the region. Methods Flow cytometry (FCM) was used to detect HPV DNA genotyping in 9 177 female genital tract specimens. The total HPV infection rate, subtype distribution, overlapping infection rate and age were statistically analyzed. Results The prevalence of HPV in this region was 16.33%, that of high-risk HPV was 11.09%, that of susceptible high-risk subtypes was HPV53 (1.37%), HPV16 (1.35%) and HPV52 The detection rate of HPV was 5.24%. The susceptible low-risk subtypes were HPV61 (1.01%) and HPV43 (0.99%). Women aged ≤20 years, 51 years old and ≥61 years old had a higher positive rate of HPV, and women ≤20 years old were predominantly infected by double infection, accounting for 45.83%. Women in all other age groups were predominantly single infections. The highest prevalence of HPV subtypes in women ≤20 years of age. Conclusion Flow-fluorescent hybridization can be used to detect HPV DNA and provide a reference for HPV vaccination and cervical cancer prevention and control in this area.