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用视网膜节细胞神经诱向因子(RGNTF)单克隆抗体及RGNTF抗独特型单克隆抗体对大鼠胚胎16～18d眼球切片进行了免疫组织化学定位及定量研究。结果表明,鼠胚视网膜节细胞的RGNTF含量从胚16～20d非常显著地减少(P<0.01),RGNTF受体的数量从胚16d到18d显著增加(P<0.05),从胚18d到20d又非常显著减少(P<0.01)。以上提示,16～18d的鼠胚视网膜节细胞既含大量RGNTF,又有大量RGNTF的受体,RGNTF对胚胎视网膜节细胞的存活及分化可能有自调节功能。20d的鼠胚视网膜节细胞的RGNTF及其受体都非常显著减少,提示节细胞在形态上分化完成后,其对RGNTF的自分泌立即显著降低,这可能是节细胞自然死亡的主要原因。
The retinal ganglion cells induced by RGNTF (RGNTF) monoclonal antibody and RGNTF anti-idiotypic monoclonal antibody were used for immunohistochemical localization and quantitative study of 16-18 day eyeballs in rat embryos. The results showed that the RGNTF content of rat retinal ganglion cells was significantly decreased from 16 to 20 days (P <0.01), and the number of RGNTF receptors increased significantly from 16 days to 18 days (P <0.05). From 18 days to 20 days Significantly decreased (P <0.01). The above tips, 16 ~ 18d rat retinal ganglion cells both contain a large number of RGNTF, there are a large number of RGNTF receptors, RGNTF embryonic retinal cells survival and differentiation may have self-regulatory function. The RGNTF and its receptor of murine embryonic retinal ganglion cells on 20d were significantly reduced, suggesting that immediately after the morphological differentiation of ganglion cells was completed, its autocrine RGNTF significantly decreased, which may be the main reason for the natural death of ganglion cells.