论文部分内容阅读
目的:证实水通道蛋白5(AQP5)在鼻息肉组织中的表达及分布。方法:挑选行鼻内镜手术的鼻息肉患者36例,分为变应组16例和非变应组20例。变应组为伴有变应性鼻炎症状患者;非变应组为不存在变应症状患者。取所有患者术后鼻息肉组织,用免疫组织化学技术检测AQP5的表达及分布,并测定阳性细胞的积分吸收度和灰度值。结果:①2组鼻息肉常规苏木精-伊红染色表现为明显的腺体增生、炎症反应和嗜酸粒细胞浸润,但变应组较非变应组更为明显;②免疫组化染色显示AQP5在变应组和非变应组鼻息肉中的分布基本一致,主要表达在腺上皮细胞、导管上皮细胞及纤毛上皮细胞的细胞膜和细胞质中;③对切片的积分吸收度进行统计学分析显示,变应组AQP5在鼻息肉细胞中的量(0.1675±0.006536)明显高于非变应组(0.09343±0.001816),2组间差异有统计学意义(P<0.01)。④对切片的灰度值进行统计学分析显示,变应组AQP5在鼻息肉细胞中的表达(175.6±2.471)明显低于非变应组(206.2±0.965),2组间差异有统计学意义(P<0.01)。结论:AQP5存在于鼻息肉组织中,在伴有变应性症状患者的鼻息肉组织中,AQP5的表达量要比非变应症状患者的鼻息肉中AQP5表达量多,推测与鼻腔分泌物的增多及其性质有关,而不同症状的鼻息肉患者可能存在不同的发病机制。
Objective: To confirm the expression and distribution of aquaporin 5 (AQP5) in nasal polyps. Methods: 36 cases of nasal polyps selected endoscopic sinus surgery were divided into 16 cases of allergic group and 20 cases of non-allergic group. Allergic group of patients with allergic rhinitis symptoms; non-allergic group of patients with allergic symptoms. The nasal polyps tissues of all patients were collected. The expression and distribution of AQP5 were detected by immunohistochemistry, and the integral absorbance and gray value of positive cells were determined. Results: ① The routine hematoxylin-eosin staining of nasal polyps in group 2 showed obvious glandular hyperplasia, inflammatory reaction and eosinophil infiltration, but the changes were more obvious in the allergic group than in the non-allergic group. ②Immunohistochemical staining showed The distribution of AQP5 in nasal polyps of allergic group and non-allergic group was basically the same, which was mainly expressed in the cell membrane and cytoplasm of glandular epithelial cells, ductal epithelial cells and ciliated epithelial cells; ③The statistical analysis of the integral absorption of the sections showed , And the amount of AQP5 in nasal polyps (0.1675 ± 0.006536) in the allergic group was significantly higher than that in the non-allergic group (0.09343 ± 0.001816). There was significant difference between the two groups (P <0.01). (4) The statistical analysis of the gray value of the slice showed that the expression of AQP5 in nasal polyps (175.6 ± 2.471) was significantly lower than that in non-allergic group (206.2 ± 0.965), the difference was statistically significant (P <0.01). CONCLUSIONS: AQP5 is present in nasal polyps. The expression of AQP5 in nasal polyps in patients with allergic symptoms is higher than that in non-allergic patients, suggesting that AQP5 is associated with increased secretion of nasal secretions Increase and its nature, and different symptoms of nasal polyps patients may have different pathogenesis.