论文部分内容阅读
目的建立雪貂感染H7N9禽流感病毒动物模型。方法 A/Anhui/1/2013(H7N9)禽流感病毒经鼻吸入感染雪貂,观察动物临床症状和体征,上呼吸道排毒情况及组织病理学变化。结果雪貂感染后出现体重下降、活动减少以及打喷嚏的临床表现,上呼吸道、心脏、肝脏及嗅球可检测到活病毒,上呼吸道排毒的峰值出现在感染后的第3~5天。血清抗体滴度最高达到1280,外周血淋巴细胞数量减少、粒细胞数量增加。组织病理学显示动物肺脏呈局灶性间质性肺炎及肺泡炎改变,CT显示肺内片状阴影。结论成功建立雪貂感染H7N9禽流感病毒的动物模型,模型的建立为H7N9禽流感发病机制研究、药物及疫苗的评价奠定了实验基础。
Objective To establish an animal model of H7N9 avian influenza virus infection in ferrets. Method A / Anhui / 1/2013 (H7N9) The bird flu virus was used to infect ferrets through nasal inhalation. The clinical symptoms and signs, the detoxification of the upper respiratory tract and the histopathological changes were observed. Results After weight loss, decreased activity and clinical manifestations of sneezing, the peak of detoxification of the upper respiratory tract was detected in the upper respiratory tract, heart, liver and olfactory bulb at 3 to 5 days after infection. Serum antibody titers up to 1280, the number of peripheral blood lymphocytes decreased, the number of granulocytes increased. Histopathology showed that the animal lung was focal interstitial pneumonia and alveolitis change, CT showed flaky lung shadow. Conclusion The animal model of H7N9 avian influenza virus infection in ferrets was successfully established. The establishment of the model laid the experimental foundation for the study on the pathogenesis of H7N9 bird flu and the evaluation of drugs and vaccines.