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目的探讨环氧合酶-2(Cyclooxygenase-2,COX-2)和低氧诱导因子-1α(hypoxiainduced factor-1α,HIF-1α)在结肠癌病变发生、发展中的作用。方法选择2010年4月—2014年12月收治的行结肠癌根治术和结肠腺瘤肿块切除术患者170例作为研究对象,根据病理结果分为A组(结肠癌组织)90例,B组(结肠腺瘤病变组织)40例,C组(癌旁正常结肠组织)40例。采用免疫组织化学法检测COX-2和HIF-1α在各组结肠组织中的表达,并分析两者与结肠癌组织临床病理特征的关系及两者间的相关性。计数资料采用χ2检验,COX-2和HIF-1α表达间的相关性采用Spearman等级相关分析,P<0.05为差异有统计学意义。结果 A组COX-2及HIF-1α的表达阳性率分别为81.1%和76.7%,显著高于B组(27.5%、22.5%)及C组(7.5%、5.0%),差异均有统计学意义(均P<0.05)。不同病理分级、侵及浆膜层、淋巴结转移及Ducks分期患者结肠癌组织中COX-2和HIF-1α的表达阳性率比较差异均有统计学意义(均P<0.05)。结肠癌组织中,COX-2和HIF-1α的阳性表达率存在显著正相关性(r=0.726,P<0.05)。结论结肠癌组织中COX-2和HIF-1α高表达且随结肠癌的病理分级、临床分期、远处转移而升高,表明COX-2和HIF-1α阳性率越高,结肠癌的恶性度越高,浸润越深。结肠癌组织中COX-2和HIF-1α的表达具有正相关关系,相互作用共同参与结肠癌的发生、发展。
Objective To investigate the role of cyclooxygenase-2 (COX-2) and hypoxia-induced factor-1α (HIF-1α) in the development of colon cancer. Methods A total of 170 patients undergoing colon cancer radical mastectomy and colon adenoma mass excision from April 2010 to December 2014 were selected as the study objects. According to the pathological findings, 90 patients were divided into group A (colon cancer), group B ( Colon adenoma lesions) 40 cases, C group (adjacent normal colorectal tissue) 40 cases. The expression of COX-2 and HIF-1α in each group of colon tissues was detected by immunohistochemistry, and the relationship between them and the clinicopathological features of colon cancer was analyzed. Counting data using χ2 test, COX-2 and HIF-1Î ± correlation between Spearman rank correlation analysis, P <0.05 for the difference was statistically significant. Results The positive rates of COX-2 and HIF-1α in group A were 81.1% and 76.7%, respectively, which were significantly higher than those in group B (27.5% and 22.5%) and group C (7.5% and 5.0% Significance (all P <0.05). The positive rates of COX-2 and HIF-1α expression in colon cancer tissues with different histological grade, invasive serosa, lymph node metastasis and Ducks stage were significantly different (all P <0.05). There was a significant positive correlation between the positive rates of COX-2 and HIF-1α in colon cancer (r = 0.726, P <0.05). Conclusions The expression of COX-2 and HIF-1α in colorectal cancer is highly expressed and increases with the pathological grade, clinical stage and distant metastasis of colon cancer, indicating that the positive rates of COX-2 and HIF-1α are higher, The higher the deeper the infiltration. There is a positive correlation between the expression of COX-2 and HIF-1α in colon cancer tissues, and the interaction is involved in the occurrence and development of colon cancer.