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应用常规瑞氏吉姆萨(G-W)染色和Feulgen反应,对银鲫外周血涂片进行了光镜观察,显微摄影后进行图像测量和分析。进一步用流式细胞仪进行了DNA含量的分析。结果显示,银鲫的红细胞在血液中存在幼稚和衰老现象,红细胞核残影广泛存在,白细胞可以区分出中性粒细胞、单核细胞、血栓细胞和淋巴细胞。嗜酸粒细胞极少,嗜碱粒细胞未被检出。Feulgen反应检测DNA含量为4.71 pg,流式细胞检测DNA含量为5.39 pg。
The peripheral blood smear of silver crucian carp was observed under light microscope with conventional Wiegand Giemsa (G-W) staining and Feulgen reaction, and the images were measured and analyzed after photomicrography. The DNA content was further analyzed by flow cytometry. The results showed that red blood cells of crucian carp had naive and senescent phenomena in the blood, erythrocyte residual images existed widely, and white blood cells could distinguish neutrophils, monocytes, thrombus and lymphocytes. Eosinophils are rare, basophils are not detected. The DNA content was 4.71 pg for Feulgen reaction and 5.39 pg for flow cytometry.