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应用聚蔗糖——泛影葡胺分层液对人胎肝细胞作非连续密度梯度分离中,CFU-C的分离效率与所用离心管的大小有关。分离的胎肝细胞经液氮低温保存三个月后,其中58—88％的CFU—C可以得到回收。
In the separation of human fetal liver cells by Ficoll-UDH, the CFU-C separation efficiency is related to the size of the centrifuge tube used. Isolated fetal liver cells were cryogenically stored in liquid nitrogen for three months, of which 58-88% of CFU-C was recovered.