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目的研究以大孔吸附树脂纯化的方法制备红花籽粕降糖有效部位的最佳工艺。方法以静态吸附法考查11种大孔树脂对有效部位的吸附和解吸附性能,以纯化后有效部位产率和酶活性数据进行综合评分,选出纯化效果最佳的树脂进行下一步动态筛选研究,并确定最佳纯化工艺。结果经过静态吸附-解吸附实验筛选出AB-8型大孔树脂为最佳纯化树脂;动态筛选结果得出最佳纯化工艺为:上样药液质量浓度为75 mg/m L;上样量为6 BV;吸附速率为3 BV/h。洗脱前先用水洗至还原糖阴性反应,再分别用10%、70%、95%乙醇以3 BV/h的速度洗脱,收集10%乙醇洗脱液6 BV和95%乙醇洗脱液5 BV,合并洗脱液,干燥后即获得有效部位。结论经AB-8型大孔树脂纯化处理后的红花籽粕有效部位降糖活性明显提高。此方法简便易行、成本低,可以用于工业化生产红花籽粕降糖有效部位。
Objective To study the best method for preparing the hypoglycemic effective part of safflower seed meal by macroporous resin purification method. Methods The static adsorption method was used to examine the adsorption and desorption properties of 11 kinds of macroporous resins to the effective sites. The yield of purified fractions and enzyme activity data were scored comprehensively to select the best purified resin for further dynamic screening. And determine the best purification process. Results The AB-8 macroporous resin was selected as the best purified resin by static adsorption-desorption experiment. The optimum purification technology was obtained by dynamic screening. The mass concentration of the sample was 75 mg / m L, 6 BV; adsorption rate of 3 BV / h. Before elution, wash with water until the reducing sugar is negative, then elute with 10%, 70%, 95% ethanol at 3 BV / h, collect 10% ethanol eluent 6 BV and 95% ethanol eluent 5 BV, the combined eluent, after drying to obtain effective site. Conclusion The hypoglycemic activity of the effective fractions of safflower seed meal purified by AB-8 macroporous resin is obviously increased. This method is simple and easy, low cost, can be used for industrial production of safflower seed meal hypoglycemic effective site.